The cDNA sequence of human NAMPT was amplified by PCR from the pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR solutions ended up digested and cloned into a pET21a+ vector using NdeI and XhoI restriction enzymes. Some extent mutation was introduced by the fast alter https://s1r-agonist-257901.mdkblog.com/32142393/the-best-side-of-dimethandrolone-undecanoate